Transferrin Receptor antibody

Catalog No. ABIN965815

This Mouse Monoclonal antibody specifically detects Transferrin Receptor in FACS, WB, IF, IHC (fro) and IHC (af). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for Transferrin Receptor antibody (ABIN965815)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Acetone-fixed) (IHC (af))

Clone: B331 (66IG10)

Product Details anti-Transferrin Receptor Antibody

Specificity: This antibody reacts with the transferrin receptor, a 180-190 kD transmembrane glycoprotein which exists as a 95 kD homodimer with interchain disulfide bond. The specificity of this antibody, as demostrated by immunoprecipitation, are equivalent to OKT9, B3/25, and BerT9.

Purification: Purified.

Immunogen: Cell line KG1.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence: 10-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunoblotting: 1-5 ug/mL.
Immunohistochemistry: 10-20 ug/mL on frozen sections using avidin-biotin system.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections. The antibody may be used at a dilution of 10-20 ug/mL in IHC.

Comment:

Recommended Positive Control: Tonsil

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-Transferrin Receptor antibody (ABIN965815)

Doenecke: "Differential response of avian red blood cell nucleosomes to heparin." in: Biochemistry international, Vol. 9, Issue 1, pp. 129-36, (1984) (PubMed).

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71

Gene ID: 7037

Pathways: Transition Metal Ion Homeostasis